The tissue-to-tissue barrier chip MEPS-TBC-CH (lower channel type), is a human tissue culture platform that allows the modeling of human physiology and pathology of key organs at a tissue level. The platform, manufactured by high-precision injection molding, comprises two microfluidic channels divided by a porous membrane. This pack of 12 chips provides 96 replicate units for high-throughput experiments.
General
This chip displays 8 identical and independent designs for 2D-2D tissue-to-tissue barrier modeling. The chip is manufactured in polystyrene and features two microfluidic channels separated by a polyester membrane with a 3 µm pore size. It is thus possible to apply physiological shear stress in both channels simultaneously. The human physiological tissue-to-tissue barrier is thus constructed under static, single, or parallel dynamic flow conditions in the apical and basolateral compartments.
The pump-free, ready-to-use platform allows high-precision sampling for drug testing and disease modeling. The inlets and outlets are compatible with a 1 µL pipette tip. A wide range of applications can be explored with the MEPS-TBC-CH, spanning from 2D vascular endothelial barrier to 2D small airway or 2D glomerular filtration barrier modeling.
Content
12x MEPS-TBC-CH chips (individually wrapped and sterile)
Specifications
| Applications |
Perfused 2D/2D tissue-tissue co-culture |
| Materials |
Polystyrene |
| Number of units per chip |
8 |
| Dimensions |
25 mm x 75 mm |
| Base |
Channel width x height |
500 µm x 500 µm |
| |
Inlet/outlet diameter |
⌀ 2.5 mm |
| |
Perfusion channel volume |
3.35 µL |
| Insert |
Channel width x height |
500 µm x 100 µm |
| |
Inlet/outlet diameter |
⌀ 2.5 mm |
| |
Perfusion channel volume |
0.3 µL |
| Membrane |
Pore size/density |
3 µm / 6E+05 cm² |
| |
Materials |
Polyester |
| |
Thickness |
9 µm |
| |
Co-culture interface surface |
1.14 mm² |
Documentation
Protocol will be provided via email after purchase.
Ahn, S. I., Sei, Y. J., Park, H. J., Kim, J., Ryu, Y., Choi, J. J., Sung, H-J., MacDonald, T. J., Levey, A. I. & Kim, Y. (2020). Microengineered human blood-brain barrier platform for understanding nanoparticle transport mechanisms. Nature Communications,11, 175. https://doi.org/10.1038/s41467-019-13896-7
Kim, J. I., Yoon, T., Kim, P., Bekhbat, M., Kang, S. M., Rho, H. S., Ahn, S. I. & Kim, Y. (2023). Manufactured tissue-to-tissue barrier chip for modeling the human blood-brain barrier and regulation of cellular trafficking. Lab on a Chip,23(13), 2990-3001. https://doi.org/10.1039/D3LC00124E
