The DuaLink Shift NeoBento™ features 8 or 16 chips per plate. The DuaLink Shift assymetrical architecture has been specially designed to create a synaptic compartment. Both channels are connected by microchannels to allow 2 independant cell culture. Neurons are used as bio-sensors, cell bodies growing in one channel and extensions in microchannels to reach and innervate any organs cultivated in the opposite channel.
The NeuroFluidics Line by NETRI is fabricated in PDMS for high transparency and biocompatibility. This technology is ideal for developing high-throughput organ-on-a-chip models to accelerate drug development and disease modeling.
General
NETRI’s NeuroFluidics Devices are innovative organ-on-a-chip platforms for organ modeling and drug discovery. They offer high-throughput data collection with up to 16 data points on the same plate. With their pump-free design and standard ANSI 96-well plate format, they can be seamlessly integrated with standard lab equipment and are compatible with a wide range of readouts for in-depth data analysis. Made in PDMS, they allow high-content imaging and continuous oxygenation. NETRI’s platforms aim to model a wide range of organs and use neurons as bio-sensors to innervate those organs.
The DuaLink Shift design of the NeuroFluidics Devices allows the culture of up to 2 cell types and synaptic isolation. It features 2 cell culture compartments each having its own media and coating solutions connected through 1 compartment for fluidic isolation. Each of the 3 channels has one inlet and one outlet.
In the DuaLink Shift architecture, the microchannels connecting the left channel (1) to the central channel (2) and connecting the central channel (2) to the right channel (3) are asymmetrical, with the length between 1-2 being smaller than 2-3 to create a synaptic compartment in the central channel (2). Central channel (2) is also smaller than channels 1 and 3 to create a fluidic isolation compartment between them, enabling interaction while isolating the cellular compartment. Because of the micron scale, only cell extensions can develop in the microchannels, while cell bodies remain in the cell compartment (channels 1,2,3).
There are two formats available:
- LIGHT: to perform up to 8 individual experiments simultaneously (half-plate)
- FULL: to perform up to 16 individual experiments simultaneously (full plate)
The technology is compatible with a wide range of readouts including ELISA, Live Staining, ImmunoFluorescence, Live/Dead Assays and more.
The DuaLink Shift platform can be used for synaptic compartmentalization, to study nerve injury & regrowth after trauma, to model Alzheimer’s disease or to visualize and quantify axonal regeneration.
Content
1x DuaLink Shift LIGHT or FULL
Specifications
| Parameters |
Channel 1 |
Channel 2 |
Channel 3 |
| Length (µm) |
18 800 |
6000 |
18 800 |
| Width (µm) |
1 000 |
200 |
1 000 |
| Height (µm) |
200 |
200 |
200 |
| Volume (µL) |
3.8 |
0.24 |
3.8 |
| Volume with inlets/outlets (µm) |
117.7 |
114.1 |
117.7 |
| Surface area (mm²) |
18.8 |
1.2 |
18.8 |
| Surface area with inlets/outlets (mm²) |
32.9 |
15.3 |
32.9 |
| Microchannels |
from 1 to 2: 100x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm
from 2 to 3: 500x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm
|
| Material |
Chip in PDMS, Structure in Polystyrene |
| Format |
Standard ANSI 96-well plate |
| Shel-life |
4 months after reception |
Documentation
DuaLink Shift technical specifications
DuaLink application note - Improve reproducibility
DuaLink application note - Surface tension-based cell seeding
DuaLink Shift application note - Synaptic transmission investigation
