The DuaLink Shift MEA NeoBento™ features 8 chips per plate. The DuaLink Shift MEA assymetrical architecture has been specially designed to create a synaptic compartment. The plate features a MicroElectrod Array (MEA) layer to record the activity of the neurons. Both channels are connected by microchannels to allow 2 independant cell culture. Neurons are used as bio-sensors, cell bodies growing in one channel and extensions in microchannels to reach and innervate any organs cultivated in the opposite channel, and record and process electrophysiological datas via MEA compatibility and NETRI's UpLink utility software.
The NeuroFluidics MEA Line by NETRI is fabricated in PDMS for high transparency and biocompatibility and is compatible with MEA-recording (Axion Maestro EDGE). This technology is ideal for developing high-throughput organ-on-a-chip models to accelerate drug development and disease modeling.
General
NETRI’s NeuroFluidics MEA Devices are MEA-capable compartmentalized organ-on-chip. They are innovative organ-on-a-chip platforms for organ modeling and drug discovery. They offer high-throughput data collection, including electrophysiology, with up to 8 data points on the same plate. With their pump-free design and standard ANSI 96-well plate format, they can be seamlessly integrated with standard lab equipment and are compatible with a wide range of readouts for in-depth data analysis. NETRI’s platforms aim to model a wide range of organs and use neurons as bio-sensors to innervate those organs.
The DuaLink Shift MEA design of the NeuroFluidics Devices allows the culture of up to 2 cell types, synaptic isolation and electrophysiology recording. It features 2 cell culture compartments each having its own media and coating solutions, connected through 1 compartment for fluidic isolation. Each of the 3 channels has one inlet and one outlet.
In the DuaLink Shift MEA architecture, the microchannels connecting the left channel (1) to the central channel (2) and connecting the central channel (2) to the right channel (3) are asymmetrical, with the length between 1-2 being smaller than 2-3 to create a synaptic compartment in the central channel (2). Central channel (2) is also smaller than channels 1 and 3 to create a fluidic isolation compartment between them, enabling interaction while isolating the cellular compartment. Because of the micron scale, only cell extensions can develop in the microchannels, while cell bodies remain in the cell compartment (channels 1,2,3).
Thanks to the MicroElectrod Array (MEA) layer, developped in collaboration with Axion BioSystems, it is possible to record the electrical activity of synapses. With 42 electrodes (white dots in the picture below) displayed both in the channels and microchannels, it is possible to record all neurons' parts: somas, axons, synapses and dendrites. It is also possible to monitor the propagation of an action potential from one channel to the other.
The electrophysiological recordings are performed thanks to Axion Biosystems' Maestro EDGE (8 chips per plate) instrument and results can be processed thanks to NETRI's Uplink software for easy interpretation of the collected data.
The plate comes in the EDGE format, displaying 8 independent designs (half-plate).
The technology is compatible with a wide range of readouts including ELISA, Live Staining, ImmunoFluorescence, Live/Dead Assays, Electrophysiology and more.
The DuaLink Shift MEA platform can be used for synaptic compartmentalization & electrical activity recording, to model Alzheimer’s disease or to model Inflammatory Bowel Disease.
Content
1x DuaLink Shift MEA EDGE
Specifications
| Parameters |
Channel 1 |
Channel 2 |
Channel 3 |
| Length (µm) |
18 800 |
6000 |
18 800 |
| Width (µm) |
1 000 |
200 |
1 000 |
| Height (µm) |
200 |
200 |
200 |
| Volume (µL) |
3.8 |
0.24 |
3.8 |
| Volume with inlets/outlets (µm) |
117.7 |
114.1 |
117.7 |
| Surface area (mm²) |
18.8 |
1.2 |
18.8 |
| Surface area with inlets/outlets (mm²) |
32.9 |
15.3 |
32.9 |
| Microchannels |
from 1 to 2: 100x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm
from 2 to 3: 500x6(±1)x3,2 µm ; LxWxH n=200; spaced by 20 µm
|
| Material |
Chip in PDMS, Structure in Polystyrene |
| Format |
Standard ANSI 96-well plate |
| Shel-life |
4 months after reception |
Documentation
DuaLink Shift MEA technical specifications
DuaLink application note - Improve reproducibility
DuaLink application note - Surface tension-based cell seeding
DuaLink MEA application note - Compartmentalized culture of primary or hiPSC-derived neurons
DuaLink Shift MEA application note - Synaptic transmission investigation
