A pack of 3 focused flow (or flow focusing) droplet generators in glass with topconnect interface to be used with the Micronit Fluidic Connect Pro chip holder.
Available uncoated (hydrophilic) for oil-in-water or with a hydrophobic coating for water-in-oil, and nozzle sizes of 10 µm, 20 µm, 50 µm or 75 µm.
General
Focused flow droplet generators are widely used in microfluidics to generate highly monodisperse droplets of various sizes. This Micronit chip offers the following features:
- Manufactured from high-quality borosilicate glass, inert to most chemicals and biocompatible
- Precision-etched microfluidic channels for reproducible results
- Three nozzle sizes: 10 µm (9-20 µm droplet range), 20 µm (20-40 µm droplet range), 50 µm (40-90 µm droplet range) or 75 µm (65-140 µm droplet range)*
- Optimized nozzle geometry: etched on both sides (oval shape), stable and highly monodisperse droplet generation
- Uncoated (hydrophilic) for oil-in-water (O/W) droplet generation, or coated (hydrophobic) for water-in-oil (W/O) droplet generation
*Indicated reference droplet sizes are based on a reference experiment with Silicone oil 5cSt and DI water combined with 2% v/v Tween 20 or Tween 80.
Double-etched droplet generator nozzle - oval channel shape
| Nozzle height |
10 µm |
20 µm |
50 µm |
75 µm |
| Nozzle width |
14 µm |
29 µm |
72 µm |
105 µm |
| Channels height (all channels except nozzle) |
17 µm |
33 µm |
83 µm |
125 µm |
| Inlet/outlet width |
367 µm |
367 µm |
433 µm |
475 µm |
| Junction channels (around the nozzle) width |
22 µm |
38 µm |
88 µm |
130 µm |
| On-chip filter size |
8-10 µm |
8-10 µm |
no filter |
no filter |
Easily change the droplet size and frequency by tuning the flow rates of the dispersed and continuous phases. These chips are suitable for foam, digital PCR, single-cell analysis, encapsulation, particle and nanoparticle production, etc.
Content
3x Focused flow drop generator - topconnect
Specifications
| |
00935 |
01341 |
02137 |
06058 |
00877 |
06011 |
00580 |
00970 |
| Nozzle height |
10 µm |
20 µm |
50 µm |
75 µm |
| Hydrophobic coating |
No |
Yes |
No |
Yes |
No |
Yes |
No |
Yes |
| Nozzle width |
14 µm |
29 µm |
72 µm |
105 µm |
| Channels height (all channels except nozzle) |
17 µm |
33 µm |
83 µm |
125 µm |
| Inlet/outlet width |
367 µm |
367 µm |
433 µm |
475 µm |
| Junction channels (around the nozzle) width |
22 µm |
38 µm |
88 µm |
130 µm |
| On-chip filter size |
8-10 µm |
8-10 µm |
no filter |
no filter |
| Chip material |
Borosilicate glass |
| Interface type |
Topconnect |
| Chip dimensions |
Supplied in a black polymer cardtridge with an external dimension of 75 x 25mm. The functional glass element has a size of 45x15mm. |
| Chip thickness |
1800 µm |
| Inlets / outlets |
2 / 1
|
| Compatible holder |
Fluidic Connect Pro |
Documentation
?FF droplet generator topconnect 10 µm - technical drawing
?FF droplet generator topconnect 50 µm - technical drawing
?FF droplet generator topconnect 75 µm - technical drawing
?Droplet generation - white paper
?How to prevent clogging?
?Using the right surfactants in your droplet generator
?Finding the right flow rates in your droplet generator
?Surface wetting properties
Amoyav, B., & Benny, O. (2018). Controlled and tunable polymer particles’ production using a single microfluidic device. Applied Nanoscience, 8(4), 905-914. https://doi.org/10.1007/s13204-018-0790-0
Lucio, A. A., Mongera, A., Shelton, E., Chen, R., Doyle, A. M., & Campàs, O. (2017). Spatiotemporal variation of endogenous cell-generated stresses within 3D multicellular spheroids. Scientific reports, 7(1), 1-11. https://doi.org/10.1038/s41598-017-12363-x
