ZEISS Primovert iLED, configured for microfluidics and cell culture
Compact inverted microscope configured by Darwin Microfluidics for daily work on microfluidic chips, droplets, and transparent samples, plus basic fluorescence using a 470 nm LED (GFP, FITC, fluorescein range). Camera not included.
General
This Primovert iLED configuration is designed for routine microfluidics workflows: chip inspection, droplet monitoring, loading checks and observation of transparent samples using phase contrast. It is also well suited for cell culture, with quick switching between brightfield, phase and fluorescence. The compact footprint makes it easy to place on crowded benches and it can fit in a laminar flow hood.
Why it works well in microfluidics
- Space where you need it: condenser 0.4 with 55 mm working distance leaves room for chips, connectors and holders. It can be removed when extra clearance is needed.
- Quick checks at the right magnification: scan at 4x, then inspect channels, droplets and regions of interest at 10x to 40x.
- Clean contrast without adjustments: one PH1 slider supports 10x, 20x and 40x, so magnification changes stay simple.
Fluorescence made simple (470 nm)
- Designed for common green fluorophores: GFP, FITC and fluorescein range.
- Switch modes on the stand: move from phase contrast to fluorescence quickly during the same session.
- LED convenience: even illumination, no warm-up, no lamp alignment, minimal maintenance.
Typical applications
- Microfluidic droplet generation and monitoring
- Chip loading verification and flow QC checks
- Observation of transparent samples using phase contrast
- GFP-labeled cells or fluorescent tracers in chips
- Routine cell culture inspection in brightfield, phase, and fluorescence
Key features
Fast to use, easy to switch contrasts, and ready for imaging workflows.
Large working area for chips
Long working distance condenser (0.4, 55 mm) provides clearance for chips, tubing and holders, while maintaining strong transmitted-light performance.
Phase contrast with one PH1 slider
PH1 supports 10x, 20x and 40x objectives, so you can change magnification without phase alignment steps.
470 nm fluorescence on demand
Basic epi-fluorescence for GFP-like fluorochromes, with quick switching from phase to fluorescence on the stand.
LED illumination for daily work
Stable color temperature, long lifetime, no alignment, and reduced sample heating compared to traditional lamps.
Flexible sample holders
Object guide and universal mounting frame support slides, dishes and many chip formats, including custom sizes.
Content
1x ZEISS Primovert iLED stand with binocular phototube (50/50) and eyepieces 10x/20
1x Transmitted-light illumination with white-light LED
1x Epi-fluorescence illumination with 470 nm LED (GFP-like fluorochromes)
1x 4-position objective nosepiece with objectives 4x, 10x Ph1, 20x Ph1, 40x Ph1
1x Long working distance condenser 0.4 with 55 mm working distance
1x 2-position phase slider (empty, Ph1)
1x Specimen stage 200 mm x 239 mm with object guide 108 mm x 72 mm, right-operated
1x Universal mounting frame for slides and dishes positioning
1x External power unit 100 to 240 VAC, 50 to 60 Hz, with country-specific adapters
1x Dust cover
Optional: Camera C-mount adapter (0.5x or 0.65x), camera not included
Specifications
Illumination
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Transmitted illumination
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White-light LED, peak wavelength 450 nm (IEC 62471 risk group 2)
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Fluorescence illumination
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Blue LED, peak wavelength 470 nm (IEC 62471 risk group 2)
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Homogeneous image field illumination
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20 mm diameter
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Brightness adjustment
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Approx. 15 to 100%
|
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Color temperature
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7,000 K (independent of brightness)
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Optics and mechanics
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Maximum field of view
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20
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Illumination
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Epi-fluorescence and transmitted light
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Fluorescence source
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LED wavelength 470 nm
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Transmitted light source
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LED 7,000 K
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Eyepiece distance (pupil distance)
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Adjustable from 48 to 75 mm
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Viewing angle
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45°
|
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Viewing height
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350 to 390 mm
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Visual output
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Tube factor 1x
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Focus drive (coarse)
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45 mm / rev
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Focus drive (fine)
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0.5 mm / rev
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Total lift
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15 mm
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Nosepiece
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4-position, manual
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Objective thread
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W 0.8
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Object stage
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Permanently installed, 200 mm x 239 mm
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Stage adjustment
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Right
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Verniers
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X-axis: number scale (read right to left); Y-axis: letter scale (read using the mirror)
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Coaxial drive
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Right
|
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LD condenser 0.4
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For objectives 4x to 40x, working distance 55 mm
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Objectives (included)
| Objective |
Numerical aperture |
Working distance |
Phase contrast |
| 4x |
0.10 |
12.0 mm |
- |
| 10x |
0.25 |
4.4 mm |
Ph1 |
| 20x |
0.30 |
4.6 mm |
Ph1 |
| 40x |
0.50 |
2.7 mm |
Ph1 |
Physical
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Dimensions (width x depth x height)
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Approx. 215.5 mm x 552 mm x 494 mm
|
|
Weight (without accessories or packaging)
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Approx. 11.5 kg
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Environmental conditions
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Transportation (in packaging) temperature
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−40°C to +70°C
|
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Storage temperature
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+10°C to +40°C
|
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Storage humidity
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Max. 75% at 35°C (without condensation)
|
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Operation area of use
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Closed spaces
|
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Operation max. altitude
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2,000 m
|
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Operation temperature
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+10°C to +40°C
|
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Operation humidity
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Max. 75% at 35°C (without condensation)
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Electrical and compliance
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Protection class
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II
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Protection type
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IP20
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Electrical safety
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Pursuant to DIN EN 61010-1 (IEC 61010-1) and in accordance with CSA and UL standards
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Degree of pollution
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2
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Overvoltage category
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II
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Radio interference suppression
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Pursuant to EN 61326-1, EN 61326-2-101
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Main voltage
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100 to 240 V (±10%); worldwide power adapter (no voltage switching required)
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Power frequency
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50/60 Hz
|
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Microscope DC
|
Adjustable 1.5 V to 6 V
|
|
LED risk group
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Risk group 2 pursuant to IEC 62471
|
Documentation
Primovert brochure
