PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 683

The PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 683 is a microfluidic chip designed to divide one liquid stream into multiple fractions with high efficiency. The device comes with a total volume of 10 chambers x 10 µl (100 µl combined), allows specific volume selection for each chamber, and features 10 individual channels. Its unique bubble-trapping rim maintains optimal conditions for fluorescent detection in PCR. Enclosed air reservoirs prevent reagent loss by evaporation, helping achieve consistent thermocycling and stable reactions.

General

These PCR chamber chips with splitter function are particularly well-suited for applications in which one microfluidic stream needs to be divided into several fractions for downstream applications. In Fluidic 683, the input is divided into ten separate 10 µl reaction chambers. Each reaction chamber is succeeded by a chamber containing an enclosed air volume, which serves to buffer volume expansion/contraction during thermocycling. Because this air volume is sealed, there is no reagent loss due to evaporation. Furthermore, Fluidic 683 features microfluidic ChipShop's unique bubble-trapping rim design, specifically developed to maintain the detection area of each chamber free from air bubbles for undisturbed fluorescent readouts. This feature makes the chip particularly suitable for use in PCR experiments, during which small air bubbles can form within the chambers of a microfluidic chip. The loading pressure is designed for 250 mBar.

Features & Benefits

• Splitter Function: Efficiently divides one liquid stream into ten equal fractions for parallel reaction setups.
• Dead-End Air Reservoirs: Each reaction chamber is connected to a sealed air volume, buffering volume changes and preventing sample evaporation during thermal cycling.
• Bubble-Trapping Rim: Proprietary rim design stops bubble interference, keeping the detection area clear for sensitive fluorescent measurements. This maintains data integrity during PCR.

The PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 683 is built for demanding microfluidic workflows, especially in PCR and similar assays. The ten-chamber layout allows parallel reactions and easy scaling for experiments requiring separation of input for downstream analysis or manipulation. Each 10 µl chamber is paired with a sealed air reservoir to stabilize sample volume during temperature changes, reducing the risk of evaporation and improving overall reaction reliability.

The chip also includes a specialized rim design to trap bubbles outside of the detection zone, a frequent problem during PCR due to temperature fluctuations and gas solubility shifts. The robust total and per-chamber capacity, transparent construction, and surface options (hydrophilized or untreated) make it adaptable for diverse lab protocols.

Applications

This chip is most useful in scientific experiments where precise, parallel division of liquids is needed. That includes PCR diagnostics, DNA amplification in microfluidic setups, and research labs handling multiple simultaneous thermal reactions. The bubble-trapping rim ensures clean fluorescent readouts even under rigorous cycling conditions.

• PCR amplification and detection
• Microfluidic parallel reactions
• Biological and chemical assay optimization

Content

1x PCR Chamber Chip Splitter Unit - With dead-end air reservoirs Fluidic 683

Specifications