Lipid Nanoparticle Synthesis Pack

Easily synthesize lipid nanoparticles (LNPs) and any organic nanoparticles with this all-in-one microfluidic pack! The bundle includes the well-known OB1 MK4 pressure controller, flow rate sensors, herringbone mixer chips, and all the necessary accessories to start from scratch and go from screening to scale-up.

Thanks to the lipid nanoparticle user guide issued by Inside Tx, you will get a clear understanding of all the intricacies of nanoparticle manufacturing through an introduction to nanoparticle synthesis, different protocols, installation instructions, and examples of characterization results

This pack is available in two versions according to the solvents and the volumes used.

General

Nanoparticle synthesis

Nanoparticles are a revolutionizing way of delivering drugs and genetic material directly to the cells thanks to their unique combination of high encapsulation efficiency, high biocompatibility, and precise targeting.

This novel technology was brought to the spotlight with the SARS-CoV-2 vaccines, where lipid nanoparticles (LNPs) were used for the intracellular delivery of mRNA, triggering an immune response from the host immune system. Outside vaccines, nanoparticles have shown immense potential in various other applications such as gene therapies (siRNA-LNP), drug delivery, or diagnostics (PLGA & liposomes).

These nanocarriers are mainly synthesized by solvent-antisolvent nanoprecipitation, caused by the rapid mixing of two phases. To encapsulate the right amount of material, minimize toxicity, and precisely target one type of cell, nanoparticle size has to be as accurate and homogeneous as possible. To ensure this, microfluidics appears as the best-suited solution as it allows for the highest level of control of the mixing conditions, leading to the optimal control of the nanoparticle critical parameters.

The microfluidic-based lipid nanoparticle synthesis pack has been assembled to allow the fine-tuning of the most important synthesis parameters of any lipidic or polymeric nanoparticle such as LNPs, liposomes, or PLGA:

• Size from 50 to 200 nm
• Size distribution (polydispersity index PDI <0.2)
• Encapsulation efficiency (EE > 95% for RNA in LNP)
• Volumes: 0,5 mL to 5L

The TFR (Total Flow Rate) is the parameter that has the most significant impact on the final size of nanoparticles, see below. It is defined as the sum of the flow rates of the aqueous phase and the organic phase. Meanwhile, the FRR (Flow Rate Ratio) is also an important parameter to adjust when using a herringbone microfluidic mixer, as it affects both the size and encapsulation efficiency of the particles. FRR is defined as the ratio between the flow rate of the aqueous phase and the flow rate of the organic phase.

This pack was created for all, from the microfluidics beginner to the nanoparticle expert. Thanks to its versatility, use one single reliable platform and adapt it to your requirements from screening to scale-up: automate the system to speed up your nanoparticle synthesis, or easily switch from low (<1 mL) to very large volume (>5L).

Lipid Nanoparticle Synthesis Pack

The lipid nanoparticle synthesis pack includes the best components on the market to efficiently and easily produce any nanoparticle. The elements that compose this comprehensive bundle were all tested and selected by InsideTx team of experts in microfluidics and nanoparticle synthesis for easy assembly and best results.

Flow control

Based on pressure-driven flow control - the most advanced flow control solutions for microfluidics - this pack offers the highest level of control of your nanoparticle synthesis conditions.

Combined with 2 flow rate sensors, it provides:

• The most precise control of the flow rate conditions, for the most homogeneous and controllable nanoparticle population
• The fastest response time for a maximized volume usage by minimizing head and tail

Two versions of the pack are available:

• Regular pack: only compatible with ethanol as a solvent, maximum total flow rate of 8 mL/min, minimum volume of 500 µL
• Advanced pack: compatible with any solvent suitable for Zeonor, maximum total flow rate of 60 mL/min, minimum volume of 1 mL

Feel free to contact us to discuss your choice of system!

Microfluidic herringbone mixer chip

This herringbone mixer chip was specially designed to synthesize nanoparticles. Both inlets lead to a mixing channel that induces chaotic flows to increase the surface of contact between the aqueous and the organic phases. This chip features multiple designs and is reusable several times if properly cleaned.

Accessories

All necessary accessories to synthesize nanoparticles are included in this pack: reservoirs, tubings, fittings, air filter, and tubing cutter. See more details in the Content tab.

User Guide

This LNP Synthesis Pack user guide includes all the information to understand nanoparticles and apprehend their synthesis. When starting from the ground up, it provides all protocols and tips to finely tune your particles, as well as a step-by-step installation manual. Remember that you have access to a team of experts in microfluidics and nanoparticle synthesis. Do not hesitate to contact us if you have any questions!

Schematics of the Regular (left) and the Advanced (right) lipid nanoparticle synthesis packs

Content

A pressure source is required to use the OB1 pressure controller (not included in the Lipid Nanoparticle Synthesis Pack).

Specifications

Pack specifications

¹ Value provided for RNA encapsulation into LNP - can change with other compounds.

Instruments & accessories specifications

OB1 Mk4 flow controller - 2 channels 0-8 bar: See full specifications here

MFS flow sensors: See full specifications here

BFS flow sensors: See full specifications here

ChipShop herringbone mixer chip Fluidic 1460: See full specifications here

Documentation

? Lipid nanoparticle synthesis pack user guide

? Microfluidic synthesis of lipid nanoparticles review